{"id":564,"date":"2026-08-11T19:51:41","date_gmt":"2026-08-11T19:51:41","guid":{"rendered":"https:\/\/syrian-biomedica.com\/aline-batch\/"},"modified":"2026-08-15T08:54:05","modified_gmt":"2026-08-15T08:54:05","slug":"aline-batch","status":"publish","type":"page","link":"https:\/\/syrian-biomedica.com\/ar\/aline-batch\/","title":{"rendered":"Aline Batch"},"content":{"rendered":"

Aline Batch<\/strong><\/h1>\n

Aline Batch a,*,1, Nizar Issa b, Khaled Mawardi c, Abdel Aleem Bello a<\/p>\n

a Dept. of Botany, Faculty of Science, University of Aleppo, Aleppo, Syria
b Dept. of Zoology, Faculty of Science, Damascus University, General Authority for Biotechnology, Damascus, Syria
c Dept. of Chemistry, Faculty of Science, University of Aleppo, Aleppo, Syria<\/h1>\n

\n

Cytotoxic and pro-apoptotic effects of Conium maculatum L. Methanolic extracts in HCT-116 colorectal cancer cells: Association with antioxidant activity<\/strong><\/p>\n<\/div>\n

\n

Conium maculatum L. (poison hemlock) is a toxic medicinal plant widely distributed in the Eastern Mediterranean region and historically recognized for its diverse biological activities. The present study aimed to characterize the phenolic profile of methanolic extracts from different plant organs and evaluate their antioxidant, cytotoxic, and pro-apoptotic activities against HCT-116 human colorectal cancer cells. Phytochemical characterization by HPLC revealed quercetin (7.86 \u00b1 0.32 mg\/100 g dry extract) and gallic acid (4.65 \u00b1 0.32 mg\/100 g dry extract) as the major phenolic constituents, accompanied by ascorbic acid, catechin, coumarin, and ferulic acid. Significant differences in total phenolic and flavonoid contents were observed among plant organs (p < 0.001), with fruits exhibiting the highest levels (30.42 \u00b1 0.09 mg GAE\/g and 28.34 \u00b1 0.085 mg QE\/g, respectively). Consistent with these findings, the fruit extract demonstrated the strongest antioxidant activity in the DPPH assay (IC\u2085\u2080 = 8 \u03bcg\/mL), followed by roots (IC\u2085\u2080 = 14 \u03bcg\/mL), leaves (IC\u2085\u2080 = 20 \u03bcg\/mL), and stems (IC\u2085\u2080 = 170 \u03bcg\/mL). Cytotoxicity assessment using the MTT assay revealed concentration-dependent inhibition of HCT-116 cell proliferation, with IC\u2085\u2080 values of 150 and 184 \u03bcg\/mL for root and aerial-part extracts, respectively, after 24 h of treatment. Flow cytometric analysis further demonstrated that the aerial-part extract exerted a pronounced pro-apoptotic effect, increasing late apoptotic cells to 21% while reducing viable cells to 76%, compared with 8% total apoptosis in cells treated with the root extract. Morphological examination confirmed characteristic apoptotic alterations, including cell shrinkage, rounding, and detachment. Collectively, these findings demonstrate that C. maculatum possesses considerable antioxidant and anticancer activities, particularly through apoptosis induction in colorectal cancer cells, and warrants further mechanistic and in vivo investigations to elucidate its active constituents and therapeutic potential.<\/p>\n<\/p>\n

Keywords<\/u>:<\/strong><\/p>\n

Conium maculatum; Phytochemical profiling; Antioxidant activity; Cytotoxicity; HCT-116 colorectal cancer cells; Apoptosis<\/p>\n<\/div>\n


\n<\/p>\n","protected":false},"excerpt":{"rendered":"

Aline Batch Aline Batch a,*,1, Nizar Issa b, Khaled Mawardi c, Abdel Aleem Bello a a Dept. of Botany, Faculty of Science, University of Aleppo, Aleppo, Syriab Dept. of Zoology, Faculty of Science, Damascus University, General Authority for Biotechnology, Damascus, Syriac Dept. of Chemistry, Faculty of Science, University of Aleppo, Aleppo, Syria Cytotoxic and pro-apoptotic […]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":"","_et_pb_custom_css":""},"class_list":["post-564","page","type-page","status-publish","hentry"],"_et_pb_custom_css":"","_links":{"self":[{"href":"https:\/\/syrian-biomedica.com\/ar\/wp-json\/wp\/v2\/pages\/564","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/syrian-biomedica.com\/ar\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/syrian-biomedica.com\/ar\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/syrian-biomedica.com\/ar\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/syrian-biomedica.com\/ar\/wp-json\/wp\/v2\/comments?post=564"}],"version-history":[{"count":3,"href":"https:\/\/syrian-biomedica.com\/ar\/wp-json\/wp\/v2\/pages\/564\/revisions"}],"predecessor-version":[{"id":595,"href":"https:\/\/syrian-biomedica.com\/ar\/wp-json\/wp\/v2\/pages\/564\/revisions\/595"}],"wp:attachment":[{"href":"https:\/\/syrian-biomedica.com\/ar\/wp-json\/wp\/v2\/media?parent=564"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}